| Solution Information | help | |
| Enzyme: | Muscarinic acetylcholine receptor M1 | |
| inhibitor: | BDBM42323 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this panel of radioligand binding assays performed by the NIMH Psychoactive Drug Screening Program (PDSP) is to determine Ki values for the NOX1 inhibitor compound SID 57287864 in selected assays from the "Late-stage radioligand binding assay to identify inhibitors of NADPH oxidase 1 (NOX1): PDSP screen Set 2" (AID 504381). Protocol Summary: A solution of the compound to be tested is prepared as a 1 mg/ml stock in buffer or DMSO according to its solubility. A similar stock of a reference compound (positive control) is also prepared. Eleven dilutions (5X assay concentration) of the test and reference compounds are prepared by serial dilution: 0.05 nM, 0.5 nM, 1.5 nM, 5 nM, 15 nM, 50 nM, 150 nM, 500 nM, 1.5 uM, 5 uM, 50 uM (thus, the corresponding assay concentrations span from 10 pM to 10 uM and include semilog points in the range where high-to-moderate affinity ligands compete with radioligand for binding sites). Radioligand is dispensed into the wel | |
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